HPLC-Determination of nine major Anthocyanins in red and rosé wines (Type-II)
OIV-MA-AS315-11 HPLC-Determination of nine major anthocyanins in red and rosé wine
Type II method
- Field of application
The analytical method concerns the determination of the relative composition of anthocyanins in red and rosé wine. The separation is performed by HPLC with reverse phase column and UV-VIS detection.
Ochratoxin A (Type-II)
OIV-MA-AS315-10 Measuring ochratoxine A in wine after going through an immunoaffinity column and HLPC with fluorescence detection
Type II method
Diethylene glycol (Type-IV)
OIV-MA-AS315-09 Diethylene glycol (2-hydoxy-ethxyethanol)
Type IV method
- Objective
Artificial Colorants (Type-IV)
OIV-MA-AS315-08 Examination of artificial colorants
Type IV method
Artificial sweeteners (TLC: saccharine, cyclamate and Dulcin) (Type-IV)
OIV-MA-AS315-07B Examination of artificial sweeteners
Type IV method
- Principle of the method
Examination of saccharine, Dulcin and cyclamate.
Artificial sweeteners (TLC : saccharine, cyclamate, Dulcin and P‑4000 ) (Type-IV)
OIV-MA-AS315-07A Examination of artificial sweeteners
Type IV method
- Principle of the method
Examination of saccharine (benzoic sulfimide), Dulcin (p-ethoxyphenylurea), cyclamate (cyclohexylsulfamate) and P‑4000 (5-nitro-2-propoxyaniline or 1propoxy-2-amino-4-nitrobenzene).
Cyanide Derivatives (Type-II)
OIV-MA-AS315-06 Cyanide derivatives
Type II method
Hydroxymethylfurfural (HPLC) (Type-IV)
OIV-MA-AS315-05B Hydroxymethylfurfural (HMF)
Type IV method
- Principle of the method
Separation through a column by reversed-phase chromatography and determination at 280 nm.
Procedures described below are given as examples.
Hydroxymethylfurfural (colorimetry) (Type-IV)
OIV-MA-AS315-05A Hydroxymethylfurfural (HMF)
Type IV method
- Principle of the method
Aldehydes derived from furan, the main one being hydroxymethylfurfural, react with barbituric acid and para-toluidine to give a red compound which is determined by colorimetry at 550 nm.
Ethyl Carbamate (Type-II)
OIV-MA-AS315-04 Ethyl carbamate
Type II method
Ethyl carbamate analysis in alcoholic beverages: selective detection method by gas chromatography/mass spectrometry
(Applicable to the determination of ethyl carbamate concentrations between 10 and 200 μg/l).