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International Methods of Analysis of Wines and Musts

 

Download the full compendium in 2 volumes (2013 edition):

                Compendium of International Methods of Analysis of Wines and Musts Vol. 1 [EN] [FR]

                Compendium of International Methods of Analysis of Wines and Musts Vol. 2 [EN] [FR]

 

Download method by method:

 

  • DEFINITIONS AND GENERAL PRINCIPLES
  • PHYSICAL ANALYSIS
  • CHEMICAL ANALYSIS
    • ORGANIC COMPOUNDS
      • SUGARS
      • ALCOHOLS
      • ACIDS
      • GAS
      • OTHER ORGANIC COMPOUNDS
    • NON-ORGANIC COMPOUNDS
      • ANIONS
      • CATIONS
      • OTHER NON-ORGANIC COMPOUNDS
  • MICROBIOLOGICAL ANALYSIS
  • OTHER ANALYSES
  • CERTIFICATES OF ANALYSIS
  • MAXIMUM ACCEPTABLE LIMITS
  • ADVICE
  • SPECIFIC METHODS FOR THE ANALYSIS OF GRAPE SUGAR (RECTIFIED CONCENTRATED MUSTS)

 

 

METHODS OF ANALYSIS FOR WINES AND MUSTS

 

  • DEFINITIONS AND GENERAL PRINCIPLES

 

Title Reference Method type  
General remarks OIV-MA-AS1-02
[EN] [FR]
Classification of analytical methods (Oeno 9/2000) OIV-MA-AS1-03
[EN] [ES] [FR]
Matrix effect for metals content analysis (Oeno 5/2000) OIV-MA-AS1-04
[EN] [ES] [FR]

 

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  • PHYSICAL ANALYSIS

 

Title Reference Method type  
Density (A 1, revised by 437/2012) OIV-MA-AS2-01A I [EN]  [FR] [ES] [DE] [IT]
Density (A 1, revised by 377/2009) OIV-MA-AS2-01B IV [EN]  [FR]   
Evaluation of the sugar content in musts, concentrated musts and grapes by refractometry (revised by 466/2012) OIV-MA-AS2-02 I [EN]  [FR] [ES] [DE] [IT]
Total dry extract (gravimetry) (A 3, revised by 377/2009 and 387/2009) OIV-MA-AS2-03A I [EN]  [FR]   
Total dry extract (densimetry) (A 3, revised by 465/2012) OIV-MA-AS2-03B IV [EN]  [FR]   
Ash (A 6, revision by 377/2009) OIV-MA-AS2-04 I [EN]  [FR]   
Alkalinity of ash (A 7, revised by 377/2009) OIV-MA-AS2-05 IV [EN]  [FR]   
Oxidation-reduction potential (Oeno 3/2000) OIV-MA-AS2-06 IV [EN] [ES] [FR]   
Chromatic characteristics (A0 revised by 377/2009) OIV-MA-AS2-07B IV [EN]  [FR]   
Wine turbidity (Oeno 4/2000, revised by 377/2009) OIV-MA-AS2-08 IV [EN]  [FR]   
Folin-Ciocalteu index (OIV Compendium ed. 1990, revised by 377/2009) OIV-MA-AS2-10 IV [EN]  [FR]   
Chromatic characteristics (Oeno 1/2006) OIV-MA-AS2-11 I [EN] [ES] [FR] [DE] [IT] 
Determination of the 18O/16O isotopic ratio of water (Oeno 353/2009) OIV-MA-AS2-12 II [EN] [ES] [FR] [DE] [IT] 
Method for the determination of the size of pieces of oak wood by screening (Oeno 406-2011) OIV-MA-AS2-13 I [EN] [ES] [FR] [DE] [IT]

 

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  • CHEMICAL ANALYSIS
    • ORGANIC COMPOUNDS
      • SUGARS

 

Title Reference Method type  
Reducing substances (A 4, revised by 377/2009) OIV-MA-AS311-01A IV [EN]  [FR]   
Glucose and fructose (enzymatic method) (revised by 377/2009) OIV-MA-AS311-02 II [EN]  [FR]   
Sugar content by HPLC (Oeno 23/2003) OIV-MA-AS311-03 II [EN] [ES] [FR]   
Stabilisation of musts to detect the addition of sucrose (A 5) OIV-MA-AS311-04
[EN]  [FR]   
Determination of the deuterium distribution in ethanol derived from fermentation of grape musts, concentrated grape musts, rectified concentrated grape musts and wines by application of nuclear magnetic resonance (SNIFNMR/ RMNFINS)
(Oeno 426-2011)
OIV-MA-AS311-05 II [EN] [ES] [FR] [DE] [IT]   
Polyols derived from sugars (Oeno 9/2006) OIV-MA-AS311-06 IV [EN] [ES] [FR] [DE] [IT] 
Glucose and fructose (pHmetry) (Oeno 10/2006, revised by 377/2009) OIV-MA-AS311-07 III [EN] [ES] [FR] [DE] [IT] 
Glucose, fructose and saccharose (pHmetry) (Oeno 11/2006, revised by 377/2009) OIV-MA-AS311-08 IV [EN] [ES] [FR] [DE] [IT] 

 

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      • ALCOHOLS

 

Title Reference Method type  
Alcoholic strength by volume (pycnometry, frequency oscillation, hydrostatic balance) (A2; 8/2000; 24/2003; revised by 377/2009) OIV-MA-AS312-01A I [EN]  [FR]   
Alcoholic strength by volume (hydrometer, refractometry) (A2, revised by 377/2009) OIV-MA-AS312-01B IV [EN]  [FR]   
Conversion tables (A2) OIV-MA-AS312-02
[EN]  [FR]   
Methanol (CG) (A 41, revised by 377/2009) OIV-MA-AS312-03A IV [EN]  [FR]   
Methanol (colorimetry) (A 41, revised by 377/2009) OIV-MA-AS312-03B IV [EN]  [FR]   
Glycerol and 2.3-butanediol (A 21, revised by 377/2009) OIV-MA-AS312-04 IV [EN]  [FR]   
Glycerol (enzymatic method) (OIV Compendium ed. 1990, revised by 377/2009) OIV-MA-AS312-05 IV [EN]  [FR]   
Determination of the isotopic ratio of ethanol (Oeno 17/2001) OIV-MA-AS312-06 II [EN] [ES] [FR]   
Glycerol (GC-C-IRMS or HPLC-IRMS method (OIV - Oeno 343-2010) OIV-MA-AS312-07 IV [EN] [ES] [FR] [DE] [IT] 

 

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      • ACIDS

 

Title Reference Method type  
Total acidity (A 10, revised by 377/2009) OIV-MA-AS313-01 I [EN] [FR]   
Volatile acidity (A 11, revised by 377/2009) OIV-MA-AS313-02 I [EN] [FR]   
Fixed acidity (A 11, revised by 377/2009) OIV-MA-AS313-03 I [EN] [FR]   
Organic acids (general HPLC method) (OIV Compendium ed. 1990, revised by 377/2009) OIV-MA-AS313-04 IV [EN] [FR]   
Tartaric acid (gravimetry) (A 12, revised by 377/2009) OIV-MA-AS313-05A IV [EN] [FR]   
Lactic acid (enzymatic method) (OIV Compendium ed. 1990, revised by 377/2009) OIV-MA-AS313-07 II [EN] [FR]   
Citric acid - chemical method (A 29) OIV-MA-AS313-08 IV [EN] [FR]   
Citric acid (enzymatic method) (OIV Compendium ed. 1990, revised by 377/2009) OIV-MA-AS313-09 II [EN] [FR]   
Total malic acid (A 33, revised by 377/2009) OIV-MA-AS313-10 IV [EN] [FR]   
L-malic acid: enzymatic method (OIV Compendium ed. 1990, revised by 377/2009) OIV-MA-AS313-11 II [EN] [FR]   
D-malic acid: enzymatic method (Oeno 6/98, revised by 377/2009) OIV-MA-AS313-12A II [EN] [FR]   
D-malic acid - enzymatic method low concentrations (Oeno 16/2002, revised by 377/2009) OIV-MA-AS313-12B IV [EN] [ES] [FR]   
L-ascorbic acid (spectrofluorimetry) (A 28, revised by 377/2009) OIV-MA-AS313-13A IV [EN] [FR]   
Sorbic acid (spectrophotometry) (A 30, revised by 377/2009) OIV-MA-AS313-14A IV [EN] [FR]   
Sorbic acid (GC) (A 30, revised by 377/2009) OIV-MA-AS313-14B IV [EN] [FR]   
Sorbic acid (TLC) (A 30, revised by 377/2009) OIV-MA-AS313-14C IV [EN] [FR]   
pH (A 31, revision by Oeno 438-2011) OIV-MA-AS313-15 I [EN] [FR]
Organic acid: ionic chromatography (Oeno 23/2004) OIV-MA-AS313-16 IV [EN] [ES] [FR] [DE] [IT]
Shikimic acid (Oeno 33/2004) OIV-MA-AS313-17 II [EN] [ES] [FR] [DE] [IT]
Sorbic acid (capillary electrophoresis) (Oeno 4/2006) OIV-MA-AS313-18 IV [EN] [ES] [FR] [DE] [IT]
Organic acids and sulphates (capillary electrophoresis) (Oeno 5/2006 extended by Oeno 407-2011) OIV-MA-AS313-19 II et III [EN] [ES] [FR] [DE] [IT]
Sorbic, benzoic, salicylic acids (Oeno 6/2006) OIV-MA-AS313-20 IV [EN] [ES] [FR] [DE] [IT] 
Metatartaric acid (Oeno 10/2007) OIV-MA-AS313-21 IV [EN] [ES] [FR] [DE] [IT] 
Determination of L-ascorbic and D-isoascorbic acids by HPLC (Oeno 11/2008) OIV-MA-AS313-22 II [EN] [ES] [FR] [DE] [IT] 
Identification of L-tartaric acid (Oeno 12/2008) OIV-MA-AS313-23 IV [EN] [ES] [FR] [DE] [IT] 

 

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      • GAS

 

Title Reference Method type  
Carbon dioxide (A 39 modified by Oeno 21/2003 and completed by resolution Oeno 3/2006, revised by 377/2009) OIV-MA-AS314-01 II [EN]  [FR]   
Method of measuring excess pressure in sparkling wines (Oeno 21/2003) OIV-MA-AS314-02 I [EN] [ES] [FR]   
Determination of the 13C/12C isotopic ratio of CO2 in sparkling wines (Oeno 7/2005) OIV-MA-AS314-03 II [EN] [ES] [FR] [DE] [IT] 
Carbon dioxide (manometric method (Oeno 2/2006) OIV-MA-AS314-04 II [EN]  [ES]  [FR]  [DE] [IT] 

 

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      • OTHER ORGANIC COMPOUNDS

 

Title Reference Method type  
Acetaldehyde (A 37, revised by 377/2009) OIV-MA-AS315-01 IV [EN] [FR]
Ethyl acetate (GC) (OIV Compendium ed. 1990, revised by 377/2009) OIV-MA-AS315-02A IV [EN] [FR]
Ethyl acetate (titrimetry) revised by 377/2009 OIV-MA-AS315-02B IV [EN] [FR]
Malvidin diglucoside (A 18, revised by 377/2009) OIV-MA-AS315-03 IV [EN] [FR]
Ethyl carbamate (Oeno 8/98, revised by 377/2009) OIV-MA-AS315-04 II [EN] [FR]
5- (Hydroxymethyl)furfural (colorimetry) (A 19, revised by 377/2009) OIV-MA-AS315-05A IV [EN] [FR]
Hydroxymethylfurfural (HPLC) (A 19, revision by 377/2009) OIV-MA-AS315-05B IV [EN] [FR]
Cyanide derivatives (Oeno 4/94, revised by 377/2009) OIV-MA-AS315-06 II [EN] [FR]
Artificial sweeteners (TLC: saccharine, cyclamate, dulcin and P-4000(A 36, revised by 377/2009) OIV-MA-AS315-07A IV [EN] [FR]
Artificial sweeteners (TLC: saccharine, cyclamate and Dulcin)  (A 36, revised by 377/2009) OIV-MA-AS315-07B IV [EN] [FR]
Artificial colourants (A 43, revised by 377/2009) OIV-MA-AS315-08 IV [EN] [FR]
Diethylene glycol (OIV Compendium ed. 1990, revised by 377/2009) OIV-MA-AS315-09 IV [EN] [FR]
Ochratoxin A (Oeno 16/2001, revised by Oeno 349-2011) OIV-MA-AS315-10 II [EN] [FR]
Determination of nine major anthocyanins in red and rosé wines using HPLC (Oeno 22/2003, Oeno 12/2007) OIV-MA-AS315-11 II [EN] [FR]
Plant proteins (Oeno 24/2004) OIV-MA-AS315-12 IV [EN] [ES] [FR] [DE] [IT] 
 
Determination of lysozyme by HPLC (Oeno 8/2007) OIV-MA-AS315-14 IV [EN] [ES] [FR] [DE] [IT] 
Determination of 3-Methoxypropane-1,2-diol and cyclic diglycerols (Oeno 11/2007) OIV-MA-AS315-15 II [EN] [ES] [FR] [DE] [IT] 
Determination of releasable 2,4,6-trichloranisole in wine (Oeno 296/2009) OIV-MA-AS315-16 IV [EN] [ES] [FR] [DE] [IT] 
Determining the presence and content of polychlorophenols and polychloroanisols (Oeno 374/2009) OIV-MA-AS315-17 IV [EN] [ES] [FR] [DE] [IT] 
Analysis of biogenic amines by HPLC (Oeno 346/2009) OIV-MA-AS315-18 II [EN] [ES] [FR] [DE] [IT] 
Glutathione content (Oeno 345/2009) OIV-MA-AS315-19 IV [EN] [ES] [FR] [DE] [IT] 
Determination of α-dicarbonyl compounds of wine by HPLC after derivation (Oeno 386A-2010) OIV-MA-AS315-20 IV [EN] [ES] [FR] [DE] [IT] 
Determination of α-dicarbonyl compounds of wine by GC after derivation (Oeno 386B-2010) OIV-MA-AS315-21 IV [EN] [ES] [FR] [DE] [IT] 
Determination of carboxymethylcellulose in white wine (Oeno 404/-2010) OIV-MA-AS315-22 IV [EN] [ES] [FR] [DE] [IT] 
Quantification of potentially allergenic residues of fining agent proteins in wine (Oeno 427-2010 modified by OIV-COMEX 502-2012) OIV-MA-AS315-23 criteria [EN] [ES] [FR] [DE] [IT] 
Determination of lysozyme in wine using high-performance capillary electrophoresis (Oeno 385/2012) OIV-MA-AS315-24 IV [EN] [FR] [ES] [DE] [IT]

 

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    • NON-ORGANIC COMPOUNDS
      • ANIONS

 

Title Reference Method type  
Total bromine  (A 23, revised by 377/2009) OIV-MA-AS321-01 IV [EN]  [FR]   
Chlorides (A 15, revision by 377/2009) OIV-MA-SA321-02 II [EN]  [FR]   
Fluorides (A22; Oeno 22/2004) OIV-MA-AS321-03 II [EN]  [FR]   
Total phosphorus  (A 16, revised by 377/2009) OIV-MA-AS321-04 IV [EN]  [FR]   
Sulphates (gravimetry) (A 14, revised by 377/2009) OIV-MA-AS321-05A II [EN]  [FR]   

 

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      • CATIONS

 

Title Reference Method type  
Ammonium (A 20, revised by 377/2009) OIV-MA-AS322-01 IV [EN]  [FR]   
Potassium (AAS) (A 8, revised by 377/2009) OIV-MA-AS322-02A II [EN]  [FR]   
Potassium (flame photometry) (A 8, revised by 377/2009) OIV-MA-AS322-02B III [EN]  [FR]   
Sodium (AAS) (A 25, revised by 377/2009) OIV-MA-AS322-03A II [EN]  [FR]   
Sodium (flame photometry) (A 25, revised by 377/2009) OIV-MA-AS322-03B III [EN]  [FR]   
Calcium (A 26, revised by 377/2009) OIV-MA-AS322-04 II [EN]  [FR]   
Iron (AAS) (A 9, revised by 377/2009) OIV-MA-AS322-05A IV [EN]  [FR]   
Iron (colorimetry) (A 9, revised by 377/2009) OIV-MA-AS322-05B IV [EN]  [FR]   
Copper (OIV Compendium ed. 1990, revised by 377/2009) OIV-MA-AS322-06 IV [EN]  [FR]   
Magnesium (A 26) OIV-MA-AS322-07 II [EN]  [FR]   
Zinc (A 45) OIV-MA-AS322-08 IV [EN]  [FR]   
Silver (OIV Compendium ed. 1990, revised by 377/2009) OIV-MA-AS322-09 IV [EN]  [FR]   
Cadmium (OIV Compendium ed. 1990, revised by 377/2009) OIV-MA-AS322-10 IV [EN]  [FR]   
Lead (criteria for methods) (Oeno 7/2006) OIV-MA-AS322-12 II [EN] [ES] [FR] [DE] [IT] 

 

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      • OTHER NON-ORGANIC COMPOUNDS

 

Title Reference Method type  
Arsenic (AAS) (Oeno 14/2002, revised by 377/2009) OIV-MA-AS323-01A IV [EN] [ES] [FR]   
Arsenic (AAS) (A 34, revised by 377/2009) OIV-MA-AS323-01B IV [EN]  [FR]   
Total nitrogen  (A 40, revised by 377/2009) OIV-MA-AS323-02A IV [EN]  [FR]   
Total nitrogen - Dumas method (Oeno 13/2002, revised by 377/2009) OIV-MA-AS323-02B II [EN] [ES] [FR]   
Boron (A 44, revised by 377/2009) OIV-MA-AS323-03 IV [EN]  [FR]   
Sulphur dioxide (titrimetry) (A 17 revised by 377/2009) OIV-MA-AS323-04A II [EN]  [FR]   
Sulphur dioxide (iodometry) (A 17 revised by 377/2009) OIV-MA-AS323-04B IV [EN]  [FR]   
Sulphur dioxide (molecular method) (A 17 revised by 377/2009) OIV-MA-AS323-04C IV [EN]  [FR]   
Sulphur dioxide - grape juice (A 17 revised by 377/2009) OIV-MA-AS323-05 IV [EN]  [FR]   
Mercury - atomic fluorescence (Oeno 15/2002, revised by 377/2009) OIV-MA-AS323-06 IV [EN] [ES] [FR]   
Multi-elemental analysis  using ICP-MS (Oeno 344-2010) OIV-MA-AS323-07 II [EN] [ES] [FR] [DE] [IT] 
Assay of pesticide residues in wine following extraction using the Quechers method
(Oeno 436/2012)
OIV-MA-AS323-08 II [EN] [FR] [ES] [DE] [IT]
 Determination of natamycin in wines (Oeno 461/2012) OIV-MA-AS323-09  IV  [EN] [FR] [ES] [DE] [IT]

 

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  • MICROBIOLOGICAL ANALYSIS

 

Title Reference Method type  
Microbiological analysis (Oeno 206-2010) OIV-MA-AS4-01 IV [EN] [ES] [FR] [DE] [IT] 
Detection of preservatives and fermentation inhibitors (Fermentability test) (A35; Oeno 6/2006; revised by 377/2009) OIV-MA-AS4-02A IV [EN] [ES] [FR] [DE] [IT] 
Detection of preservatives and fermentation inhibitors (Detection of sorbic, benzoic, p-chlorobenzoic, salicylic acids and p-hydrobenzoic acid and its esters) (A35; Oeno 6/2006; revised by 377/2009) OIV-MA-AS4-02B IV [EN] [FR]
Detection of preservatives and fermentation inhibitors (Detection of monohalogen derivatives of acetic acid) (A35; Oeno 6/2006; revised by 377/2009) OIV-MA-AS4-02C IV [EN] [FR]
Detection of preservatives and fermentation inhibitors (Detection and determination of ethyl pyrocarbonate) (A35; Oeno 6/2006; revised by 377/2009) OIV-MA-AS4-02D IV [EN] [FR]
Detection of preservatives and fermentation inhibitors (Detection of dehydroacetic acid) (A35; Oeno 6/2006; revised by 377/2009) OIV-MA-AS4-02E IV [EN] [FR]
Detection of preservatives and fermentation inhibitors (Sodium azide by HPLC) (A35; Oeno 6/2006; revised by 377/2009) OIV-MA-AS4-02F IV [EN] [FR]
Enumerating yeasts of the species Brettanomyces bruxellensis using qPCR (Oeno 414-2011) OIV-MA-AS4-03 IV [EN] [ES] [FR] [DE] [IT]

 

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  • OTHER ANALYSES

 

Title Reference Method type  
Differentiation of fortified musts and sweet fortified wines (revised by 377/2009) OIV-MA-AS5-01
[EN]  [FR]   

 

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  • CERTIFICATES OF ANALYSIS

 

Title Reference Method type  
Rules for the application of methods of analysis OIV-MA-B1-01
[EN]  [FR]   
Certificates of analysis templates OIV-MA-B1-02
[EN]  [FR]   

 

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  • MAXIMUM ACCEPTABLE LIMITS

 

Title Reference Method type  
Maximum acceptable limits of various elements contained in wine OIV-MA-C1-01
[EN]  [FR]   

 

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  • ADVICE

 

Title Reference Method type  
Gluconic acid (Oeno 4/91) OIV-MA-D1-01
[EN]  [FR]   
Characterisation of wines resulting from overpressing (Oeno 5/91) OIV-MA-D1-02
[EN]  [FR]   
Level of sodium and chloride ions in wines (Oeno 6/91) OIV-MA-D1-03
[EN]  [FR]   

 

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  • SPECIFIC METHODS FOR THE ANALYSIS OF GRAPE SUGAR (RECTIFIED CONCENTRATED MUSTS)

 

Title Reference Method type  
Conductivity (Oeno 419A-2011) OIV-MA-F1-01 IV [EN] [FR] [ES] [IT] [DE]
Hydroxymethylfurfural (HMF) by High-Performance Liquid Chromatography (Oeno 419A-2011) OIV-MA-F1-02 IV [EN] [FR] [ES] [IT] [DE]
Determination of the acquired alcoholic strength by volume (ASV) of concentrated musts (CM) and grape sugar (or rectified concentrated musts, RCM) (Oeno 419A-2011) OIV-MA-F1-03 IV [EN] [FR] [ES] [IT] [DE]
Sucrose by high-performance liquid chromatography (Oeno 419A-2011) OIV-MA-F1-04 IV [EN] [FR] [ES] [IT] [DE]
Total  acidity (Oeno 419A-2011) OIV-MA-F1-05 IV [EN] [FR] [ES] [IT] [DE]
pH (Oeno 419A-2011) OIV-MA-F1-06 IV [EN] [FR] [ES] [IT] [DE]
Sulphur dioxide (Oeno 419A-2011) OIV-MA-F1-07 IV [EN] [FR] [ES] [IT] [DE]
Chromatic characteristics (Oeno 419A-2011) OIV-MA-F1-08 IV [EN] [FR] [ES] [IT] [DE]
Specific methods for the analysis of grape sugar:   total cations (Oeno 419B/2012) OIV-MA-F1-09 I [EN] [FR] [ES] [DE] [IT]
Specific methods for the analysis of grape sugar :  heavy metal by ETAAS (Oeno 419B/2012) OIV-MA-F1-10 IV [EN] [FR] [ES] [DE] [IT]
Specific methods for the analysis of grape sugar:  heavy metal (ICP-MS) (Oeno 419B/2012) OIV-MA-F1-11 IV [EN] [FR] [ES] [DE] [IT]

 

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